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iodixanol gradient optiprep  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc iodixanol gradient optiprep
    Iodixanol Gradient Optiprep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iodixanol+gradient+optiprep/iodixanol+optiprep/pm40091430-121-13-16
    Average 90 stars, based on 1 article reviews
    iodixanol gradient optiprep - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active.
    Article Snippet: .. Stained SF-EVs were further purified overnight by bottomup density gradient centrifugation using a iodixanol gradient (OptiPrep, StemCELL Technologies, Cat. No. 07820). ..

    Article Title: Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active
    Article Snippet: .. Stained SF‐EVs were further purified overnight by bottom‐up density gradient centrifugation using a iodixanol gradient (OptiPrep, StemCELL Technologies, Cat. No. 07820). ..

    Purification:

    Article Title: Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active.
    Article Snippet: .. Stained SF-EVs were further purified overnight by bottomup density gradient centrifugation using a iodixanol gradient (OptiPrep, StemCELL Technologies, Cat. No. 07820). ..

    Article Title: Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active
    Article Snippet: .. Stained SF‐EVs were further purified overnight by bottom‐up density gradient centrifugation using a iodixanol gradient (OptiPrep, StemCELL Technologies, Cat. No. 07820). ..

    Gradient Centrifugation:

    Article Title: Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active.
    Article Snippet: .. Stained SF-EVs were further purified overnight by bottomup density gradient centrifugation using a iodixanol gradient (OptiPrep, StemCELL Technologies, Cat. No. 07820). ..

    Article Title: Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active
    Article Snippet: .. Stained SF‐EVs were further purified overnight by bottom‐up density gradient centrifugation using a iodixanol gradient (OptiPrep, StemCELL Technologies, Cat. No. 07820). ..

    other:

    Article Title: MicroRNA-29a-3p prevents Schistosoma japonicum-induced liver fibrosis by targeting Roundabout homolog 1 in hepatic stellate cells.
    Article Snippet: Isolation of primary HSCs, hepatocytes, and Kupffer cells The liver was initially digested in situ with 0.05% collagenase type IV at 37 °C with bath shaking for 30 min. Hepatocytes were isolated via centrifugation of the resulting cell suspension at 50×g for 3 min and purified via centrifugation at 50×g for 2 min. After hepatocytes were pelleted, the supernatant containing nonparenchymal cells was centrifuged at 450×g for 8 min. HSCs were isolated from nonparenchymal cells using a 15% (w/v) and 11.5% (w/v) iodixanol gradient (OptiPrep; Stemcell Technologies, Vancouver, British Columbia, Canada) at 1450×g for 20 min. To further purify HSCs, we depleted HSCs of Kupffer cells (KCs) using a biotin-conjugated For continuous variables, the results are expressed as medians and interquartile ranges.



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    (A) Schematic of the ACE2 and ACE2-GPI constructs used in this study. For ACE2-GPI, the transmembrane domain and cytoplasmic tail of ACE2 were replaced with Thy1, a GPI-anchored protein, to localize ACE2 to lipid rafts. (B) Surface expression of ACE2 and ACE2-GPI by flow cytometry analysis by ACE2 surface staining with anti-ACE2 VHH-B07-Fc. A serial dilution of ACE2-expression vector was used to transfect 293T cells before surface staining. Shown is the total percentage of ACE2-positive cells (left) and representative contour plots taken from one independent repeat (right). Data are means ±s.d. of three independent experiments. (C) 293T cells expressing ACE2 or ACE2-GPI were incubated with recombinant biotinylayed-SARS-CoV-2 RBD (Wuhan variant). Cell surface staining with streptavidin AF-488 was carried out at indicated timepoints. Data were normalized to 0h post-RBD staining. Data are means ±s.d. of three independent experiments. (D) Treatment of 293T cells with phospholipase C at indicated concentrations for 2 hours at 37°C. ACE2 surface levels were assessed by flow cytometry. Data were normalized to NT conditions. Data are means ±s.d. of three independent experiments. (E) Histogram plots from flow cytometry analysis of ACE2 cell surface staining of 293T cells (left) and MRC-5 cells (right) stably expressing ACE2 or ACE2-GPI. Results are representative of at least three independent experiments. (F) Cell lysates of 293T cells (above) and MRC-5 cells (below) stably expressing ACE2 or ACE2-GPI were ultracentrifuged in an <t>iodixanol</t> gradient to isolate membranes on their lipid content. * = Lipid raft associated protein, flotillin-1, was used as a control for lipid raft localization. For B, C, and D, Mann-Whitney tests were performed to compare ACE2 and ACE2-GPI conditions, * P < 0.05, ** P < 0.001, *** P < 0.0001, ns = not significant.
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    Image Search Results


    (A) Schematic of the ACE2 and ACE2-GPI constructs used in this study. For ACE2-GPI, the transmembrane domain and cytoplasmic tail of ACE2 were replaced with Thy1, a GPI-anchored protein, to localize ACE2 to lipid rafts. (B) Surface expression of ACE2 and ACE2-GPI by flow cytometry analysis by ACE2 surface staining with anti-ACE2 VHH-B07-Fc. A serial dilution of ACE2-expression vector was used to transfect 293T cells before surface staining. Shown is the total percentage of ACE2-positive cells (left) and representative contour plots taken from one independent repeat (right). Data are means ±s.d. of three independent experiments. (C) 293T cells expressing ACE2 or ACE2-GPI were incubated with recombinant biotinylayed-SARS-CoV-2 RBD (Wuhan variant). Cell surface staining with streptavidin AF-488 was carried out at indicated timepoints. Data were normalized to 0h post-RBD staining. Data are means ±s.d. of three independent experiments. (D) Treatment of 293T cells with phospholipase C at indicated concentrations for 2 hours at 37°C. ACE2 surface levels were assessed by flow cytometry. Data were normalized to NT conditions. Data are means ±s.d. of three independent experiments. (E) Histogram plots from flow cytometry analysis of ACE2 cell surface staining of 293T cells (left) and MRC-5 cells (right) stably expressing ACE2 or ACE2-GPI. Results are representative of at least three independent experiments. (F) Cell lysates of 293T cells (above) and MRC-5 cells (below) stably expressing ACE2 or ACE2-GPI were ultracentrifuged in an iodixanol gradient to isolate membranes on their lipid content. * = Lipid raft associated protein, flotillin-1, was used as a control for lipid raft localization. For B, C, and D, Mann-Whitney tests were performed to compare ACE2 and ACE2-GPI conditions, * P < 0.05, ** P < 0.001, *** P < 0.0001, ns = not significant.

    Journal: bioRxiv

    Article Title: SARS-CoV-2 entry and fusion are independent of ACE2 localization to lipid rafts

    doi: 10.1101/2024.07.13.603361

    Figure Lengend Snippet: (A) Schematic of the ACE2 and ACE2-GPI constructs used in this study. For ACE2-GPI, the transmembrane domain and cytoplasmic tail of ACE2 were replaced with Thy1, a GPI-anchored protein, to localize ACE2 to lipid rafts. (B) Surface expression of ACE2 and ACE2-GPI by flow cytometry analysis by ACE2 surface staining with anti-ACE2 VHH-B07-Fc. A serial dilution of ACE2-expression vector was used to transfect 293T cells before surface staining. Shown is the total percentage of ACE2-positive cells (left) and representative contour plots taken from one independent repeat (right). Data are means ±s.d. of three independent experiments. (C) 293T cells expressing ACE2 or ACE2-GPI were incubated with recombinant biotinylayed-SARS-CoV-2 RBD (Wuhan variant). Cell surface staining with streptavidin AF-488 was carried out at indicated timepoints. Data were normalized to 0h post-RBD staining. Data are means ±s.d. of three independent experiments. (D) Treatment of 293T cells with phospholipase C at indicated concentrations for 2 hours at 37°C. ACE2 surface levels were assessed by flow cytometry. Data were normalized to NT conditions. Data are means ±s.d. of three independent experiments. (E) Histogram plots from flow cytometry analysis of ACE2 cell surface staining of 293T cells (left) and MRC-5 cells (right) stably expressing ACE2 or ACE2-GPI. Results are representative of at least three independent experiments. (F) Cell lysates of 293T cells (above) and MRC-5 cells (below) stably expressing ACE2 or ACE2-GPI were ultracentrifuged in an iodixanol gradient to isolate membranes on their lipid content. * = Lipid raft associated protein, flotillin-1, was used as a control for lipid raft localization. For B, C, and D, Mann-Whitney tests were performed to compare ACE2 and ACE2-GPI conditions, * P < 0.05, ** P < 0.001, *** P < 0.0001, ns = not significant.

    Article Snippet: Lysates were gently mixed with 2 mL of 60% OptiPrep iodixanol density gradient medium (Sigma-Aldrich) within 15 mL ultracentrifuge tubes.

    Techniques: Construct, Expressing, Flow Cytometry, Staining, Serial Dilution, Plasmid Preparation, Incubation, Recombinant, Variant Assay, Stable Transfection, Control, MANN-WHITNEY